《植物生理学报》 2010, 46(5): 466-470

百脉根嫁接方法的优化及其在结瘤自调控研究中的应用

李晓琳¹, 孙杰^1,*, 陈爱民², 王彦章²

1 石河子大学农学院, 石河子大学新疆生产建设兵团绿洲生态农业重点实验室, 新疆石河子832000; 2 中国科学院上海生命科学研究院植物生理生态研究所, 植物分子遗传国家重点实验室, 上海200032

通信作者：孙杰；E-mail: sunjie@shzu.edu.cn；Tel: 0993-20 572 01

摘　要：

嫁接试验是研究结瘤自调控的有效方法。本文优化了百脉根的嫁接方法, 并应用该方法初步研究了百脉根突变体rel3根瘤数目减少的机理。结果表明, rel3突变体根瘤数目减少的表型是由来自于茎的信号决定。

关键词：百脉根; 结瘤自调控; 嫁接

收稿：2009-12-30   修定：2010-04-12

资助：新疆生产建设兵团绿洲生态农业重点实验室开放课题(200503)和“973” 项目(2010CB126501)。

Optimization of a Grafting Method of Lotus japonicus R. and Its Application in Autoregulation of Nodulation

LI Xiao-Lin¹, SUN Jie^1,*, CHEN Ai-Min², WANG Yan-Zhang²

¹College of Agriculture, Shihezi University/Key Laboratory of Oasis Eco-agriculture, Xinjiang Production and Construction Group,Shihezi, Xinjiang 832000, China; ²National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Corresponding author: SUN Jie; E-mail: sunjie@shzu.edu.cn; Tel: 0993-20 572 01

Abstract:

Grafting is one of effective approaches to study autoregulation of nodulation. A grafting method of Lotus japonicus was modified and the mechanism of decrease in nitrogen-fixing nodules of L. japonicus rel3 mutant was invesigated. The results indicated that the phenotype of reduced nodule number of rel3 mutant was in control of shoot-derived signals.

Key words: Lotus japonicus; autoregulation of nodulation; grafting